Cristian Romero and Lisandro Martinez could not meet their Argentinian teammates in Miami ahead of a clash with Honduras.
According to TyC Sports, Premier League centre-backs were unable to register their visas with the US Embassy in London after it was closed following the death of Queen Elizabeth II.
The pair, who play for Tottenham and Manchester United respectively, still have a chance to link with the side before their World Cup squad is announced in November, with visas being processed in Argentina instead.
The pair of centre-backs will almost certainly feature in Lionel Scaloni’s final squad for the trip to Qatar as both have already impressed for their clubs this season.
Martinez has adapted well to the Premier League since his high-profile move from Ajax and Romero has become a key figure at the heart of Spurs’ defence, alongside recalled England defender Eric Dier.
TyC reports that the pair will now have to travel to their home country to have their visas processed before they can enter the US and until then they will be training at the Argentine FA headquarters.
Argentina meet Honduras on Saturday and the two are set to be available for selection in that clash after predicting they could be on a plane to Miami on Wednesday.
In their second game of the international break, they meet Jamaica in New Jersey on Wednesday 28 September in what will also be their last confirmed game ahead of the World Cup opener on 22 November against Saudi Arabia.
Lionel Messi, Angel di Maria, Leandro Paredes, Nicolas Tagliafico and Paulo Dybala have all joined the squad ahead of the Florida international break.
Messi is sure to contribute to his 162 caps in Qatar – injuries permitting – and the seven-time Ballon d’Or winner seems to be back at his best, playing alongside Kylian Mbappe and Neymar for PSG.
The former Barcelona talisman already has 14 goals contributions (six goals, eight assists) in just 11 appearances in all previous competitions in 2022-23.
At 35, the 2022 World Cup could be the little wizard’s last and, having inspired his side to the Brazil 2014 final, he will surely want to go one better to add an extra jewel to his already glittering career.